Efectividad antimicrobiana del aceite esencial de menta al 25, 50 y 100% frente a cepas de porphyromonas gingivalis. Estudio in vitro
The purpose of this research work was to verify the inhibitory effect of the essential oil of the leaves of Mint in concentrations of 25%, 50% and 100% and at 24, 48 and 72 hours on Porphyromona Gingivalis because the essential oil of Mint or better known as Peppermint, it has oxygenated monoterpen...
Gorde:
| Egile Nagusiak: | , , |
|---|---|
| Formatua: | Artikulua |
| Hizkuntza: | Gaztelania |
| Argitaratua: |
2021
|
| Gaiak: | |
| Sarrera elektronikoa: | https://dialnet.unirioja.es/servlet/oaiart?codigo=7946000 |
| Baliabidea: | RECIMUNDO: Revista Científica de la Investigación y el Conocimiento, ISSN 2588-073X, Vol. 5, Nº. 2, 2021, pags. 307-315 |
| Etiketak: |
Etiketa erantsi
Etiketarik gabe: Izan zaitez lehena erregistro honi etiketa jartzen
|
| Laburpena: |
The purpose of this research work was to verify the inhibitory effect of the essential oil of the leaves of Mint in concentrations of 25%, 50% and 100% and at 24,
48 and 72 hours on Porphyromona Gingivalis because the essential oil of Mint or better known as Peppermint, it has oxygenated monoterpenes responsible for
its biological activity such as: astringent, carminative, antiseptic, stimulating, anodyne, spasmolytic, deworming and antimicrobial Desarm et al. In his research
he verified the antimicrobial activity of Peppermint on human pathogenic microorganisms such as S. aureus and S. epidermidis. The methodological design of
this research was experimental in vitro, analytical, observational and longitudinal. The sample consisted of 50 white filter paper discs, which were distributed
as follows: 10 discs for each concentration of the essential oil 25%, 50% and 100%. In addition, 10 discs for 0.12% chlorhexidine as positive control and 10
discs for distilled water used as negative control; in total 5 groups. In addition, three measurements were made at three evaluation moments at 24, 48 and
72 hours. The procedure that was carried out to obtain the essential oil was through the collection of the raw material in the province of Manabí, transferred
to the laboratories of the Faculty of Agricultural Sciences of the Universidad Laica Eloy Alfaro de Manabí, the dry leaves were dried For 3 hours in the stove
at 60 ºC, to obtain the Essential Oil of Mint, the steam distillation technique was used, this being the most used for the extraction of essential oils from various
medicinal plants. A certified distillation equipment was used in which it was kept at a temperature of 25ºC to 30ºC in order to avoid losses of essential oil
due to volatilization. After obtaining it, 10 discs were placed in each Petri dish soaked in essential oil in concentrations of 25%, 50% and 100%, 10 discs for
negative control and 10 discs for positive control, at a temperature of 37 ° C; The study was analyzed in a time of 24, 48 and 72 hours, therefore, the inhibition
halos were measured at each time with the millimeter caliper, using the Duraffourd Scale. Statistically, the normality test was carried out in order to channel the
statistics to the parametric or non-parametric dimension. The Kolmogorov test with Lilliefors correction (n <30) or the Shapiro Wilks test was used. Descriptive
statistics (mean and standard deviation) were estimated. Likewise, in order to determine whether the numerical differences evidenced for the inhibition halo
were statistically significant, the Kruskall Wallis test was carried out, as well as the U Mann Whitney test. Once the entire investigative process had been carried
out, it was found that the essential oil of Mint presents inhibitory characteristics on Porphyromona Gingivalis, being the highest average in concentrations of
100% with an average of 13 mm for said concentration, located at a very sensitive level of the scale. Chlorhexidine presented a standard halo, so it can be
shown that the halo of the 100% oil exceeded that of 0.12% Chlorhexidine at 72 hours. The inhibitory capacity for the experimental groups increased over time. |
|---|